My home brew L-Carnitine.....

[Lordallmighty]

Wich filter was it that was recomended? did batch 2 few days ago and for some reason it was alot harder to filter, almost dident work. its 13mm (dident crystalize this time, when adding ba pre powder)

Should i go for 33mm ones? incase the 25 something isent big enough. some write alot of product are lost in the bigger filters

 

[01dragonslayer]

Wich filter was it that was recomended? did batch 2 few days ago and for some reason it was alot harder to filter, almost dident work. its 13mm (dident crystalize this time, when adding ba pre powder)

Should i go for 33mm ones? incase the 25 something isent big enough. some write alot of product are lost in the bigger filters

I've have always used the 13mm Whatmans. That's typical of the 13s....fthey are harder to use than others due to differences in thickness, pore size, and wet strength,

Filters with smaller pore sizes and higher thickness (e.g., Grade 3 or 542) are slower to use but retain more particles, while faster filters (e.g., Grade 4) are easier for coarse precipitates but clog more easily with fine or viscous samples.

 

[Lordallmighty]

I've have always used the 13mm Whatmans. That's typical of the 13s....fthey are harder to use than others due to differences in thickness, pore size, and wet strength,

Filters with smaller pore sizes and higher thickness (e.g., Grade 3 or 542) are slower to use but retain more particles, while faster filters (e.g., Grade 4) are easier for coarse precipitates but clog more easily with fine or viscous samples.

Hmm interesting. thanks

Btw why do you think a solution thats crystal clear when brewed, a few days later becomes abit cloudy?

 

[Lordallmighty]

Do anyone know how much bicarbonate to add in order to raise PH ?

Its very strange that nad+ took 5-10 min to fix the PH, had to throw it away multiple times due to how fast it was vs carnitine. became to alkaline. But this last batch with carnitine i spent at least a hour adding n adding bicarbonate yet zero difference on ph. I tought my ph sticks were bunk as they never changed colour. how is this even possible?

(nad is 5x smaller amount so will oviously take shorter time but this was insaine)

 

[TomJ]

Wich filter was it that was recomended? did batch 2 few days ago and for some reason it was alot harder to filter, almost dident work. its 13mm (dident crystalize this time, when adding ba pre powder)

Should i go for 33mm ones? incase the 25 something isent big enough. some write alot of product are lost in the bigger filters

33mm PES .22um filters are the way to go

 

[TomJ]

Do anyone know how much bicarbonate to add in order to raise PH ?

Its very strange that nad+ took 5-10 min to fix the PH, had to throw it away multiple times due to how fast it was vs carnitine. became to alkaline. But this last batch with carnitine i spent at least a hour adding n adding bicarbonate yet zero difference on ph. I tought my ph sticks were bunk as they never changed colour. how is this even possible?

You don't want to use bicarb to adjust pH.
You want NaOH reagent grade to raise pH and reagent grade HCL to lower.

However, if you're just making 600mg/ml carn from something like bulk supplements carnitine, you should t need to adjust the pH at all.

600mg/ml in sterile WFI with 1.5% BA should come out at around a 7.5 without any adjusting

 

[01dragonslayer]

Hmm interesting. thanks

Btw why do you think a solution thats crystal clear when brewed, a few days later becomes abit cloudy?

A clear injectable peptide becomes cloudy a few days later primarily because the peptide is not fully dissolved and has started to precipitate out of the solution, or it has degraded or possible contamination. Other causes include exposure to heat, which can accelerate degradation, or issues with the original peptide powder, such as impurities or being a large, aggregating protein.

 

[Lordallmighty]

You don't want to use bicarb to adjust pH.
You want NaOH reagent grade to raise pH and reagent grade HCL to lower.

However, if you're just making 600mg/ml carn from something like bulk supplements carnitine, you should t need to adjust the pH at all.

600mg/ml in sterile WFI with 1.5% BA should come out at around a 7.5 without any adjusting

Arent NaOH the chemical thats toxic to inhale, how can that be safe for injection?

My carnitine is analyzed so its pure and correct monequle, no acetyl or taurate, but the store bought pre homebrewing hurted to bad i had to throw it away. whatever ph carnitine is as its sulfur, its way to low, prob the compound with most complains so it seems like something everyone experience

How much NaOH do you usually add when doing a 100cc x 500-600mg/ml solution?

 

[Lordallmighty]

You don't want to use bicarb to adjust pH.
You want NaOH reagent grade to raise pH and reagent grade HCL to lower.

However, if you're just making 600mg/ml carn from something like bulk supplements carnitine, you should t need to adjust the pH at all.

600mg/ml in sterile WFI with 1.5% BA should come out at around a 7.5 without any adjusting

Bro what is this shit, it sounds even worse then i tought! haha

"The most important adverse physicochemical, human health and environmental effects: Skin corrosion produces an irreversible damage to the skin; namely, visible necrosis through the epidermis and into the dermis."

"May be corrosive to metals Causes severe skin burns and eye damage"

"Wear protective gloves/protective clothing/eye protection/face protection"

"Following skin contact After contact with skin, wash immediately with plenty of water. Immediate medical treatment required because corrosive injuries that are not treated are hard to cure."

"Sodium hydroxide technical grade, a toxic substance and hazardous chemical. This may be used as a strong base in chemical solutions"

No thanks i rather not inject that IM

 

[TomJ]

Bro what is this shit, it sounds even worse then i tought! haha

"The most important adverse physicochemical, human health and environmental effects: Skin corrosion produces an irreversible damage to the skin; namely, visible necrosis through the epidermis and into the dermis."

"May be corrosive to metals Causes severe skin burns and eye damage"

"Wear protective gloves/protective clothing/eye protection/face protection"

"Following skin contact After contact with skin, wash immediately with plenty of water. Immediate medical treatment required because corrosive injuries that are not treated are hard to cure."

"Sodium hydroxide technical grade, a toxic substance and hazardous chemical. This may be used as a strong base in chemical solutions"

No thanks i rather not inject that IM

I think maybe homebrewing isn't for you

 

[TomJ]

Pure sodium hydroxide is an extremely strong base.

Touching pure sodium hydroxide will cause chemical burns.

We are talking about adjusting the pH with 1-2 grams for an entire liter.
That is a very highly diluted solution and is perfectly safe.

But it doesn't matter for l carnitine specifically because carnitine does not need adjustment up.

By default 600mg/ml comes out only slightly above neutral. If you wanted to balance it, it would require an acid (HCL) not a base.

 

[Icyries]

My carnitine burns after sc injection. Also I get small lumps, is it normal or did I do something wrong? I followed the guide.

 

[FearsomeCOR]

My carnitine burns after sc injection. Also I get small lumps, is it normal or did I do something wrong? I followed the guide.

I wouldn’t pin carn subq

 

[TomJ]

My carnitine burns after sc injection. Also I get small lumps, is it normal or did I do something wrong? I followed the guide.

This is normal, l carnitine has to high osmality for subQ at regular concentrations.

It's better IM

 

[01dragonslayer]

My carnitine burns after sc injection. Also I get small lumps, is it normal or did I do something wrong? I followed the guide.

This is normal, l carnitine has to high osmality for subQ at regular concentrations.

It's better IM

The osmolarity of 'typical' or 'regular' L-carnitine 500 mg/mL injectable solution is around 2158 mOsm/L (or 2.158 mOsm/mL).
This high osmolarity means the solution is significantly hypertonic compared to human blood plasma, which is about 300-310 mOsm/L.

Sub Q injections generally requires solutions to be closer to isotonic (around 300 mOsm/kg) or no more than 600 mOsm/kg to minimize pain, irritation, and tissue damage. A 500 mg/mL solution is likely to cause some pain and tissue irritation if injected subcutaneously without dilution due to its high osmolarity.

 

[Lordallmighty]

The osmolarity of 'typical' or 'regular' L-carnitine 500 mg/mL injectable solution is around 2158 mOsm/L (or 2.158 mOsm/mL).
This high osmolarity means the solution is significantly hypertonic compared to human blood plasma, which is about 300-310 mOsm/L.

Sub Q injections generally requires solutions to be closer to isotonic (around 300 mOsm/kg) or no more than 600 mOsm/kg to minimize pain, irritation, and tissue damage. A 500 mg/mL solution is likely to cause some pain and tissue irritation if injected subcutaneously without dilution due to its high osmolarity.

Saw someone mention that 200mg/ml are closer to isotonic, do you remember how those injections felt like? was almost 10 years ago i used 200mg/ml

Ive started to realize im building up scar tissue from my injections. stopped for a few weeks and today i did 1cc (600mg) with 1cc b12. but it still sting pretty bad. want to use these for the rest of my life so getting scar tissue just a month in is a big no no. i wonder why bicarb dosent help better with ph

Feels pointless doing a new batch with 150mg/ml or something

 

[Lordallmighty]

Saw someone mention that 200mg/ml are closer to isotonic, do you remember how those injections felt like? was almost 10 years ago i used 200mg/ml

Ive started to realize im building up scar tissue from my injections. stopped for a few weeks and today i did 1cc (600mg) with 1cc b12. but it still sting pretty bad. want to use these for the rest of my life so getting scar tissue just a month in is a big no no. i wonder why bicarb dosent help better with ph

Feels pointless doing a new batch with 150mg/ml or something

Think im gonna dilute the bottle i have and make a 150mg/ml solution (plus use bicarbonate n see if it helps). Pretty surpriced how well the 600mg worked today + 2g oral acetyl. wayy better mental energy and look fuller. must be really deficiant in it

Strange as i dident feel 800mg before and needed 1.2g + to feel it but after a break it was much easier to notice, and the look in the mirror

Will try 3cc x 150mg per day ala 550mg + oral

 

[Lordallmighty]

Pure sodium hydroxide is an extremely strong base.

Touching pure sodium hydroxide will cause chemical burns.

We are talking about adjusting the pH with 1-2 grams for an entire liter.
That is a very highly diluted solution and is perfectly safe.

But it doesn't matter for l carnitine specifically because carnitine does not need adjustment up.

By default 600mg/ml comes out only slightly above neutral. If you wanted to balance it, it would require an acid (HCL) not a base.

btw, how can something that causes chemical burns aka low ph, makes carnitine create a high ph solution?

 

[Farmerdan]

btw, how can something that causes chemical burns aka low ph, makes carnitine create a high ph solution?

Its a base, it has a high ph. Acids have low ph

 

[TomJ]

btw, how can something that causes chemical burns aka low ph, makes carnitine create a high ph solution?

You should have probably not slept through chemistry in highschool.

Naoh is a strong base, meaning very high pH, this also causes chemical burns.

Have you ever seen fight club?
Pouring the lye on the back of the hand?
That's a strong base.

Regardless, carnitine doesn't need anything to balance its pH. Carnitine is only slightly alkaline naturally, so standard 500-600mg/ml concentrations come.out to 7.5-8pH meaning no adjustment necessary. You would never use naoh or sodium bicarbonate or any other alkaline substance to raise the pH of l carnitine.

 

[clandestino]

You should have probably not slept through chemistry in highschool.

Naoh is a strong base, meaning very high pH, this also causes chemical burns.

Have you ever seen fight club?
Pouring the lye on the back of the hand?
That's a strong base.

Regardless, carnitine doesn't need anything to balance its pH. Carnitine is only slightly alkaline naturally, so standard 500-600mg/ml concentrations come.out to 7.5-8pH meaning no adjustment necessary. You would never use naoh or sodium bicarbonate or any other alkaline substance to raise the pH of l carnitine.

Would stronger concentrations of l carnitine need to be adjusted?

 

[TomJ]

Would stronger concentrations of l carnitine need to be adjusted?

Potentially, but stronger concentrations come with their own issues.

The highest you can push a stable solution without solvents is 600-700mg/ml normally.

Going much higher than that becomes extremely thick with a very high osmality.
Meaning it's gonna be a bitch to draw, a bitch to pin, and is gonna have pip no matter what you do with the pH.

 

[Lordallmighty]

I fucking love this stuff, works so much better then MOTS-C

Finally got the PH right. Changed the mg as well to 300mg/ml instead of 600mg/ml, much less pain and easier to change ph. dont need 2g per day around 900-1200mg per day is enough

Raised the ph to high last time and basically felt zero pain after injected. great! but had to look up how fast it breaks down as the ph became to high and it said hours. maby not correct but the day after i felt basically nothing. usually feel a dopamine high and increase in energy right away

So i throw out the rest yesterday and made another batch, not making it go over 7. tought it would sting alot worse but it felt really good as well. great... will run this for the rest of my life. best drug ever

Getting ss31 this week, i hope it works better then carnitine otherwise i will just buy more carnitine and stop the expencive mito peptides

 

[Lordallmighty]

Do you guys add all the powder right away n just let the magnetic stirrer dissolve it?

Ive done both and i do belive the last time i added it all tougheter n just left it there for 10 mins or something and it all became clear. Did the same with NAD powder and 30 mins later nothing dissolved, threw it out and added few grams in a row - dissolved

Soon to do carnitine again and i dont remember how i did, dont wanna throw away an entire batch

 

[Vee]

I am totally digging injectable Carnitine

 

[Sinwolfsigma]

This is fifth batch i have made few months ago, first and 2nd vial is 600mg/ml and 3rd vial is 700mg/ml, the first almost at the end is crystal-clear however the 2nd vial got little hazy. The 3rd vial is little bit cloudy or has crystallised l carnitine in it I believe. Are the 2nd and 3rd vial good to use or should I throw them out and make a new ones?

 

[Sinwolfsigma]

This is fifth batch i have made few months ago, first and 2nd vial is 600mg/ml and 3rd vial is 700mg/ml, the first almost at the end is crystal-clear however the 2nd vial got little hazy. The 3rd vial is little bit cloudy or has crystallised l carnitine in it I believe. Are the 2nd and 3rd vial good to use or should I throw them out and make a new ones?

@01dragonslayer

 

[01dragonslayer]

@01dragonslayer

There are a few things you can do to prior to pitching your time and effort in the trash.

Let me ask you first....when you brewed your 2nd and 3rd batches did you reuse any of your supplies....like a mixing vial, needles or syringes? Sometimes the smallest impurities will cause variations in an end product? Did you brew your 2nd and 3rd as you did your 1st?

Are you able to post up some pics?

 

[Sinwolfsigma]

There are a few things you can do to prior to pitching your time and effort in the trash.

Let me ask you first....when you brewed your 2nd and 3rd batches did you reuse any of your supplies....like a mixing vial, needles or syringes? Sometimes the smallest impurities will cause variations in an end product? Did you brew your 2nd and 3rd as you did your 1st?

Are you able to post up some pics?

Thanks for reply man.

I have reattached the pics , just to give a background my house got on fire in first week of March even though the fire didn't come to my room but fumes were there on the room. Although these vials were covered and in the closet. The first vial and 2nd vial were crystal-clear even after few days of this incident however the 2nd one became little hazy.

Both the vials were of the same batch I made 200ml and put into 2 100 ml vials. For the 3rd vial, which is 700mg (without sticker) I didn't clean the beaker as it had last batch of 600mg but it didn't have any product in it but probably the residue thn I added NaCl water injection use and heated for 30 secs in microwave and followed your recipe after it.

 

[Sinwolfsigma]

Thanks for reply man.

I have reattached the pics , just to give a background my house got on fire in first week of March even though the fire didn't come to my room but fumes were there on the room. Although these vials were covered and in the closet. The first vial and 2nd vial were crystal-clear even after few days of this incident however the 2nd one became little hazy.

Both the vials were of the same batch I made 200ml and put into 2 100 ml vials. For the 3rd vial, which is 700mg (without sticker) I didn't clean the beaker as it had last batch of 600mg but it didn't have any product in it but probably the residue thn I added NaCl water injection use and heated for 30 secs in microwave and followed your recipe after it.

The 3rd vial has been same from the beginning didn't change even after that incident