Vacuum Filtering

[Throwaway_00000]

Does anyone have a tutorial for vacuum filtering? Not filtering any AAS just some L-Carnitine, glutathione, NAD, and 5 amino. Really the issue is the glutathione and l carnitine as they are extremely think and even with a caulk gun it is tedious

 

[TomJ]

filtering isn't the part you need to solve, that's simple, just requires extra equipment.
pump
media bottles
autoclave (ideally)

it's the filling that's the part you have to solve.
how do you plan on getting your now sterile filtered product into a sterile vial?
now you need a flow hood.


tldr, if you want to switch to vacuum filtration, there's extra steps and extra equipment you're going to need if you want to do it in a sterile way

 

[Bolan]

Easy......
You'll need some beakers to do your mixing
Buy some presealed vials.... 50 ml
Millipore filter and flask- A brake bleeder at the local parts store
Or the one that GPZ has.
And some 60ml Syringes

All can be bought @ GPZ .

GPZMedLab - America's Choice For Online Medical & Laboratory Supplies

GPZ Med Lab carries all the best in Medical supplies, Laboratory Supplies, and Compounding Supplies. Glass Beakers, Media Bottles, Hypodermic Syringes and Needles, Millipore Steritops, Foxx Autovials, Nalgene Filter Units, Alcohol Prep Pads, Unsealed Amber and Clear Serum Vials, Presealed...

www.gpzmedlab.com

 

[jpe1]

https://www.sigmaaldrich.com/US/en/product/aldrich/z222593

Just screw this on a media bottle and use a brake bleeder pump but like Tom said then you'll need to figure out how to transfer while staying sterile.

Unless you are large scaling caulk gun is easy and can set and forget it. Have this attachment. Irwin 6" Quick Grip Syringe Adapter - Water Gun by fox_socks_

 

[Throwaway_00000]

filtering isn't the part you need to solve, that's simple, just requires extra equipment.
pump
media bottles
autoclave (ideally)

it's the filling that's the part you have to solve.
how do you plan on getting your now sterile filtered product into a sterile vial?
now you need a flow hood.


tldr, if you want to switch to vacuum filtration, there's extra steps and extra equipment you're going to need if you want to do it in a sterile way

Yes that's the part that was confusing me. I figured it would introduce contaminants without filtering directly into a sealed vial. Is there a cheap way to make a flow hood?

 

[Bolan]

Yes that's the part that was confusing me. I figured it would introduce contaminants without filtering directly into a sealed vial. Is there a cheap way to make a flow hood?

You do not need a laminar flow hood unless your brewing for the board.
Once filtered suck it up with the 60ml and attach an 18g dart and squirt it into the pre sealed and sterile vials.

Its so easy really.

 

[Throwaway_00000]

You do not need a laminar flow hood unless your brewing for the board.
Once filtered suck it up with the 60ml and attach an 18g dart and squirt it into the pre sealed and sterile vials.

It's so easy really.

Am I just overthinking this? Let's say I bought this kit here:

Labfil Laboratory Vacuum Filtration Distillation Apparatus, Borosilicate Glass Vacuum Filtration Assembly Filter Kit with 500ML Filtering Flask and 300ML Graduated Funnel: Amazon.com: Industrial & Scientific

Labfil Laboratory Vacuum Filtration Distillation Apparatus, Borosilicate Glass Vacuum Filtration Assembly Filter Kit with 500ML Filtering Flask and 300ML Graduated Funnel: Amazon.com: Industrial & Scientific

a.co

And just bought replacement 0.22um filters. Would that work?

 

[Bolan]

Am I just overthinking this? Let's say I bought this kit here:

Labfil Laboratory Vacuum Filtration Distillation Apparatus, Borosilicate Glass Vacuum Filtration Assembly Filter Kit with 500ML Filtering Flask and 300ML Graduated Funnel: Amazon.com: Industrial & Scientific

Labfil Laboratory Vacuum Filtration Distillation Apparatus, Borosilicate Glass Vacuum Filtration Assembly Filter Kit with 500ML Filtering Flask and 300ML Graduated Funnel: Amazon.com: Industrial & Scientific

a.co

And just bought replacement 0.22um filters. Would that work?

If there is a .22 that fits it. then you'll need some presealed sterile vials.
60ml Syringes a few of 18g needles.
Just get it all at GPZ. plus the milipores are not $70 for the flask and receiver.

 

[TomJ]

Yes that's the part that was confusing me. I figured it would introduce contaminants without filtering directly into a sealed vial. Is there a cheap way to make a flow hood?

short answer, no.
that's one corner you don't want to cheap out on or cut corners on.

if you want to use a vacuum filter to handle the bulk of the filtering, you can do so and then still syringe filter into sterile vials like you would normally.

it will be a ton easier and faster after it's been filtered.

this will eliminate the need for an autoclave and flow hood.

 

[TomJ]

Yes that's the part that was confusing me. I figured it would introduce contaminants without filtering directly into a sealed vial. Is there a cheap way to make a flow hood?

short answer, no.
that's one corner you don't want to cheap out on or cut corners on.

if you want to use a vacuum filter to handle the bulk of the filtering, you can do so and then still syringe filter into sterile vials like you would normally.

it will be a ton easier and faster after it's been filtered.

this will eliminate the need for an autoclave and flow hood.


all this being said, if you're just brewing for personal use, you can decide for yourself how risk tolerant you want to be.
I have had an abscess before and will for sure be doing my damnest to make sure I don't give myself another one

 

[Throwaway_00000]

short answer, no.
that's one corner you don't want to cheap out on or cut corners on.

if you want to use a vacuum filter to handle the bulk of the filtering, you can do so and then still syringe filter into sterile vials like you would normally.

it will be a ton easier and faster after it's been filtered.

this will eliminate the need for an autoclave and flow hood.


all this being said, if you're just brewing for personal use, you can decide for yourself how risk tolerant you want to be.
I have had an abscess before and will for sure be doing my damnest to make sure I don't give myself another one

So like a double filter? Maybe one filter through a 0.22 vacuum filter into a beaker then a 0.4 syringe into a sterile vial?? My reasoning is that it wouldn't need to be put through the smallest again just to filter out anything that might have gone in between vials

 

[TomJ]

So like a double filter? Maybe one filter through a 0.22 vacuum filter into a beaker then a 0.4 syringe into a sterile vial?? My reasoning is that it wouldn't need to be put through the smallest again just to filter out anything that might have gone in between vials

if anything at all, the opposite.
the very last step should be .22um filter.

you could think of it as "pre-filtering" with the vacuum pump to remove the majority of the large solids and contaminants that clog up and slow down your syringe filters

 

[Throwaway_00000]

if anything at all, the opposite.
the very last step should be .22um filter.

you could think of it as "pre-filtering" with the vacuum pump to remove the majority of the large solids and contaminants that clog up and slow down your syringe filters

I understand my reasoning was that the 0.22 would be the hardest to filter through, so I would want the vacuum there and then just hand filter through a 0.4 into a sterile vial so that it would flow quickly and wouldn't be too hard on my thumbs

 

[TomJ]

I understand my reasoning was that the 0.22 would be the hardest to filter through, so I would want the vacuum there and then just hand filter through a 0.4 into a sterile vial so that it would flow quickly and wouldn't be too hard on my thumbs

get a caulk gun, filtering through the .4 would be pointless

 

[davidboy]

https://www.sigmaaldrich.com/US/en/product/aldrich/z222593

Just screw this on a media bottle and use a brake bleeder pump but like Tom said then you'll need to figure out how to transfer while staying sterile.

Unless you are large scaling caulk gun is easy and can set and forget it. Have this attachment. Irwin 6" Quick Grip Syringe Adapter - Water Gun by fox_socks_

Once you filter gear how you transfer from media bottle to sealed 10ml vials? Thansk

 

[userID1234]

I understand my reasoning was that the 0.22 would be the hardest to filter through, so I would want the vacuum there and then just hand filter through a 0.4 into a sterile vial so that it would flow quickly and wouldn't be too hard on my thumbs

Found a syringe press on Etsy that would probably be easier than a caulk gun. Looks like you can use a ratchet with it too

https://cactuslovercreations.etsy.com/listing/4530886423/manual-syringe-pumppress-for-easy-tube