HPLC purity vs. identity testing

[mangopeachyy]

I'm trying to understand what the different tests on a peptide COA actually tell you.
My understanding is that HPLC is used to assess purity, while mass spectrometry can help confirm the compound's identity. But neither result alone tells you everything about sterility, endotoxins, or the actual amount in a vial.
Is that distinction correct? I'd be interested in any reliable resources that explain how to interpret these results properly.

 

[Knarfster]

I'm trying to understand what the different tests on a peptide COA actually tell you.
My understanding is that HPLC is used to assess purity, while mass spectrometry can help confirm the compound's identity. But neither result alone tells you everything about sterility, endotoxins, or the actual amount in a vial.
Is that distinction correct? I'd be interested in any reliable resources that explain how to interpret these results properly.

Sterility and Endo Toxins are extra for tests. And the peptide test should tell you the amount in the vial.

 

[mangopeachyy]

Sterility and Endo Toxins are extra for tests. And the peptide test should tell you the amount in the vial.

Thanks, that clears it up. I was mixing up purity and actual content, so I'll pay attention to those as separate results.