Rubber stopper sterilisation (help please)

Page 1 of 2 12 LastLast
Results 1 to 15 of 23
  1. #1
    Banned


    Join Date
    Jun 2017
    Posts
    130

    Thanks Thanks Given 
    0
    Thanks Thanks Received 
    6
    Thanked in
    6 Posts
    Rep Points
    -214149574

    Rubber stopper sterilisation (help please)

    I want to Sterile rubber stoppers for Vials so please post how do u sterile rubber stoppers?

  2. #2
    Senior Member
    Btheman's Avatar


    Join Date
    Mar 2017
    Posts
    589

    Thanks Thanks Given 
    99
    Thanks Thanks Received 
    98
    Thanked in
    82 Posts
    Rep Points
    1973692457

    I soak mine in alcohol the put in the oven for two hours on 200 degrees just like my vials .

  3. #3
    Banned


    Join Date
    Jun 2017
    Posts
    130

    Thanks Thanks Given 
    0
    Thanks Thanks Received 
    6
    Thanked in
    6 Posts
    Rep Points
    -214149574

    Quote Originally Posted by Btheman View Post
    I soak mine in alcohol the put in the oven for two hours on 200 degrees just like my vials .
    And no problem? Like infection or melt rubber stoppers? U make sterilisation with dry heat?

  4. #4
    Senior Member
    Murse32's Avatar


    Join Date
    Nov 2016
    Posts
    247

    Thanks Thanks Given 
    1
    Thanks Thanks Received 
    6
    Thanked in
    5 Posts
    Rep Points
    1997584637

    I use an Olizee portable steam autoclave (on Amazon for $195). I put my vials, rubber stoppers, and flip top caps in a sterilization pouch and autoclave for 45 minutes.


    Sent from my iPhone using Tapatalk

  5. #5
    Banned


    Join Date
    Jun 2017
    Posts
    130

    Thanks Thanks Given 
    0
    Thanks Thanks Received 
    6
    Thanked in
    6 Posts
    Rep Points
    -214149574

    Quote Originally Posted by Murse32 View Post
    I use an Olizee portable steam autoclave (on Amazon for $195). I put my vials, rubber stoppers, and flip top caps in a sterilization pouch and autoclave for 45 minutes.


    Sent from my iPhone using Tapatalk
    Is it good? How u pack them? (Vials and rubber stoppers) with foil or... Also what do u use for sterilisation in automotive? Distilled water?

  6. #6
    Senior Member
    Murse32's Avatar


    Join Date
    Nov 2016
    Posts
    247

    Thanks Thanks Given 
    1
    Thanks Thanks Received 
    6
    Thanked in
    5 Posts
    Rep Points
    1997584637

    Just regular tap water. Any microorganisms in the water will be killed during steam sterilization.

    I put 25 vials, 25 rubber stoppers, and 25 flip top caps in these pouches:

    https://www.amazon.com/gp/aw/d/B00ON...236_SY340_QL65


    Sent from my iPhone using Tapatalk

  7. #7
    Banned


    Join Date
    Jun 2017
    Posts
    130

    Thanks Thanks Given 
    0
    Thanks Thanks Received 
    6
    Thanked in
    6 Posts
    Rep Points
    -214149574

    Quote Originally Posted by Murse32 View Post
    Just regular tap water. Any microorganisms in the water will be killed during steam sterilization.

    I put 25 vials, 25 rubber stoppers, and 25 flip top caps in these pouches:

    https://www.amazon.com/gp/aw/d/B00ON...236_SY340_QL65


    Sent from my iPhone using Tapatalk
    Do u put one vial with rubber in one punch or all Vials and rubbers in one punch? Also how u wash Vials and rubbers stoppers before put in the autoclave? Please post autoclave link

  8. #8
    Senior Member
    Murse32's Avatar


    Join Date
    Nov 2016
    Posts
    247

    Thanks Thanks Given 
    1
    Thanks Thanks Received 
    6
    Thanked in
    5 Posts
    Rep Points
    1997584637

    Quote Originally Posted by Gio View Post
    Do u put one vial with rubber in one punch or all Vials and rubbers in one punch? Also how u wash Vials and rubbers stoppers before put in the autoclave? Please post autoclave link
    Dude. 25 vials, stoppers, and caps in one pouch. I'll sterilize two pouches at the same time. Once the sterilizer gets hot enough, it will blow steam out of the safety valve. That's when you start your timer for 45 minutes. After that, unplug the sterilizer and crack the lid. Let the pouches cool. They will be wet; it's important not to let them touch anything while they are wet! Bacteria can move through the water on the surface of the pouch. I wear sterile gloves and pull them out of the sterilizer and place them on to a sterile drape when they are done to avoid this.

    https://www.amazon.com/gp/aw/d/B073R...qOL&ref=plSrch


    Sent from my iPhone using Tapatalk

  9. #9
    Banned


    Join Date
    Jun 2017
    Posts
    130

    Thanks Thanks Given 
    0
    Thanks Thanks Received 
    6
    Thanked in
    6 Posts
    Rep Points
    -214149574

    Quote Originally Posted by Murse32 View Post
    Dude. 25 vials, stoppers, and caps in one pouch. I'll sterilize two pouches at the same time. Once the sterilizer gets hot enough, it will blow steam out of the safety valve. That's when you start your timer for 45 minutes. After that, unplug the sterilizer and crack the lid. Let the pouches cool. They will be wet; it's important not to let them touch anything while they are wet! Bacteria can move through the water on the surface of the pouch. I wear sterile gloves and pull them out of the sterilizer and place them on to a sterile drape when they are done to avoid this.

    https://www.amazon.com/gp/aw/d/B073R...qOL&ref=plSrch


    Sent from my iPhone using Tapatalk
    Thank u such a good answer. Also can u help me and tell which bottle top filter u use for filtration? Which brand... I use neglete bottle top filter 250ml 0.2um is it good. I need them for testosterone and tren a. Which u use? Sorry. My broken English

  10. #10
    Senior Member
    Murse32's Avatar


    Join Date
    Nov 2016
    Posts
    247

    Thanks Thanks Given 
    1
    Thanks Thanks Received 
    6
    Thanked in
    5 Posts
    Rep Points
    1997584637

    Quote Originally Posted by Gio View Post
    Thank u such a good answer. Also can u help me and tell which bottle top filter u use for filtration? Which brand... I use neglete bottle top filter 250ml 0.2um is it good. I need them for testosterone and tren a. Which u use? Sorry. My broken English
    I like these.

    https://www.amazon.com/gp/aw/d/B00BU...236_SY340_QL65


    Sent from my iPhone using Tapatalk

  11. #11
    Banned


    Join Date
    Jun 2017
    Posts
    130

    Thanks Thanks Given 
    0
    Thanks Thanks Received 
    6
    Thanked in
    6 Posts
    Rep Points
    -214149574

    Quote Originally Posted by Murse32 View Post
    I like these.

    https://www.amazon.com/gp/aw/d/B00BU...236_SY340_QL65


    Sent from my iPhone using Tapatalk
    This is PES membrane. Is it good for solutions with ba bb? I read that PES doesn't good for oils. Nylon is good. Do u use bottle tops with PES membrane?

  12. #12
    Senior Member
    Murse32's Avatar


    Join Date
    Nov 2016
    Posts
    247

    Thanks Thanks Given 
    1
    Thanks Thanks Received 
    6
    Thanked in
    5 Posts
    Rep Points
    1997584637

    Quote Originally Posted by Gio View Post
    This is PES membrane. Is it good for solutions with ba bb? I read that PES doesn't good for oils. Nylon is good. Do u use bottle tops with PES membrane?
    I use these filters for all my solutions. All I usually use is 2% BA, 15% BB and MCT oil with my powders. I've never had an infection.


    Sent from my iPhone using Tapatalk

  13. #13
    Senior Member
    Btheman's Avatar


    Join Date
    Mar 2017
    Posts
    589

    Thanks Thanks Given 
    99
    Thanks Thanks Received 
    98
    Thanked in
    82 Posts
    Rep Points
    1973692457

    Quote Originally Posted by Gio View Post
    And no problem? Like infection or melt rubber stoppers? U make sterilisation with dry heat?
    I use dry heat because moisture crashes your gear. Never had a problem or infection . At 200 the will not melt .

  14. #14
    Banned


    Join Date
    Jun 2017
    Posts
    130

    Thanks Thanks Given 
    0
    Thanks Thanks Received 
    6
    Thanked in
    6 Posts
    Rep Points
    -214149574

    Quote Originally Posted by Murse32 View Post
    I use these filters for all my solutions. All I usually use is 2% BA, 15% BB and MCT oil with my powders. I've never had an infection.


    Sent from my iPhone using Tapatalk
    OK I'll buy this bottle tops and autoclave. And how many ml water do u use for autoclave and how u use autoclave?

  15. #15
    Banned


    Join Date
    Jun 2017
    Posts
    130

    Thanks Thanks Given 
    0
    Thanks Thanks Received 
    6
    Thanked in
    6 Posts
    Rep Points
    -214149574

    Quote Originally Posted by Btheman View Post
    I use dry heat because moisture crashes your gear. Never had a problem or infection . At 200 the will not melt .
    Moisture? I talk about empty Vials. Dry heat machine is cheaper but. Autoclave is better. I only filter gear and put them in the Steril Vials. Filtration also sterilize gear. Do u put gear in dry heat?

Similar Threads

  1. Vial Beaker rubber stopper sterilisation
    By Gio in forum Anabolic Steroids
    Replies: 23
    Last Post: 06-27-2017, 05:00 AM
  2. Sterilisation - Vials beakers. Rubber stopper and Ect
    By Gio in forum Research & Homebrew Chemistry
    Replies: 2
    Last Post: 06-25-2017, 02:39 PM
  3. Floater in test cypionate from rubber stopper
    By Cavnasty in forum Anabolic Steroids
    Replies: 10
    Last Post: 05-09-2016, 04:50 PM
  4. Drawing with an 18g, puncture in silicone/ rubber stopper
    By CalorieKiller22 in forum Anabolic Steroids
    Replies: 3
    Last Post: 08-09-2015, 06:55 PM

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
Copyright© 2012-2021 Anabolic Steroid Discussion Forums