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How to sterilize vials

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    Post How to sterilize vials



    Oven
    Dry heat is less effective than steam for sanitizing and sterilizing, but many brewers use it. The best place to do dry heat sterilization is in your oven. To sterilize an item, refer to the following table for temperatures and times required.

    Table 3 - Dry Heat Sterilization

    Temperature Duration

    338°F (170°C) 60 minutes

    320°F (160°C) 120 minutes

    302°F (150°C) 150 minutes

    284°F (140°C) 180 minutes

    250°F (121°C) 12 hours (Overnight)

    The times indicated begin when the item has reached the indicated temperature. Although the durations seem long, remember this process kills all microorganisms, not just most as in sanitizing. To be sterilized, items need to be heat-proof at the given temperatures. Glass and metal items are prime candidates for heat sterilization.

    Some homebrewers bake their bottles using this method and thus always have a supply of clean sterile bottles. The opening of the bottle can be covered with a piece of aluminum foil prior to heating to prevent contamination after cooling and during storage. They will remain sterile indefinitely if kept wrapped.

    One note of caution: bottles made of soda lime glass are much more susceptible to thermal shock and breakage than those made of borosilicate glass and should be heated and cooled slowly (e.g. 5 °F per minute). You can assume all beer bottles are made of soda lime glass and that any glassware that says Pyrex or Kimax is made of borosilicate.


    Autoclaves and Pressure Cookers

    Typically when we talk about using steam we are referring to the use of an autoclave or pressure cooker. These devices use steam under pressure to sterilize items. Because steam conducts heat more efficiently, the cycle time for such devices is much shorter than when using dry heat. The typical amount of time it takes to sterilize a piece of equipment in an autoclave or pressure cooker is 20 minutes at 257° F (125 °C) at 20 pounds per square inch (psi).

    BUY STERILE SEALED VIALS
    You can also buy sealed vials and remove the aluminum cap and then use a syringe needle to vent the stopper and fill will a syringe with a 18 or 15g needle Then recap. This is more expensive to do but if you are only doing it at a small scale then this maybe best for you.

    Please Vet brewers add Ideas to this thread to help noobs keep there glass vials sterile.

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    Learn something new everyday

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    Quote Originally Posted by jonboy View Post
    Learn something new everyday
    You will learn a lot from my posts my friend.

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    Is it enough to do it in sterilizer designed to sterilize bottles for newborns?

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    Quote Originally Posted by szatas View Post
    Is it enough to do it in sterilizer designed to sterilize bottles for newborns?
    In my honoest opinion, No. Baby bottle sterilizers kills bacterias that baby's stomach acid cant kill, and leaving anything that may be killed by baby's stomach acid. But sterilizing injectable are TOTALY different story.

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    Quote Originally Posted by Liquidex View Post
    In my honoest opinion, No. Baby bottle sterilizers kills bacterias that baby's stomach acid cant kill, and leaving anything that may be killed by baby's stomach acid. But sterilizing injectable are TOTALY different story.

    ^^this.
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    how can you sterilize rubber stoppers also?

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    Quote Originally Posted by JJR View Post
    how can you sterilize rubber stoppers also?
    First dip them in 10% bleach, then leanse with 70% rubbing alcohol. Pharmasuitacle companies do it by radio sterilization and dont even have to touch alcohol, but obviously we cant do that unless you have accesse to the nuclear machine.

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    How to sterilize vials

    Quote Originally Posted by Liquidex View Post
    First dip them in 10% bleach, then leanse with 70% rubbing alcohol. Pharmasuitacle companies do it by radio sterilization and dont even have to touch alcohol, but obviously we cant do that unless you have accesse to the nuclear machine.
    And they need to dry, it's the process of drying that allows the alcohol to kill the bacteria.
    And 70% alcohol is the most effective, that's why it's the most common.

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    would dipping the rubber into 70% alcohol then covering then until dry be effective? Im just trying to figure out all the steps and how to xactly do it.

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    couldnt you bake the stoppers? at 340 degrees for 1 hour as with the glass weer I dont know that 340 is enough to distort them.

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    Quote Originally Posted by JJR View Post
    couldnt you bake the stoppers? at 340 degrees for 1 hour as with the glass weer I dont know that 340 is enough to distort them.
    I am not sure about heating the rubber stoppers. They may melt you know. I would just use 70% rubbing alcohol. Should eliminate 99.99% of bacteria.

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    How to sterilize vials

    Quote Originally Posted by Liquidex View Post
    I am not sure about heating the rubber stoppers. They may melt you know. I would just use 70% rubbing alcohol. Should eliminate 99.99% of bacteria.
    You can bake them at 250 for about 30 minutes after a IPA bath

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    Quote Originally Posted by colochine View Post
    You can bake them at 250 for about 30 minutes after a IPA bath
    Hmm interesting. Thanks colochine. Ill try that right now

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    What is the best time to sterilize vials?
    I mean should I fill vials shortly after sterilization or I can do it much later?

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    Quote Originally Posted by szatas View Post
    What is the best time to sterilize vials?
    I mean should I fill vials shortly after sterilization or I can do it much later?
    As long as you KNOW its sterile, you can fill up right away. But I like to make vials to be completely dry before filling em up

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    THANK YOU for posting this! you on fire with good shit bro! lol now i just need to get everything i need

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    Question... In the Homebrew Walkthrough sticky, his glass isnt covered with anything when he is mixing/stirring/ect.. Should it be covered when heating and mixing the powder with the oil and everything? If so, when stirring, what is the best/safest way of doing this? Is it ok to uncover the glass? if so, how long can it stay uncovered?

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    following this intently as I'm interested in learning more about home brewing. Thanks for posting

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    Quick update.

    If you dont have time to wait 2 to 4 hours with 300'F heat, do the following method.

    1. Wash ALL the vials with 70% isoprophyl alcohol.

    2. In to the oven and set to 550"F and bake for 1 hour.

    Its now Hospital grade (usp grade) sterile.

    Fairly simple. But if your oven doesnt go this high, just go buy portable one that goes as high temperature.

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