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This one boggles my mind.
You need:
- Powder weight
- Solvent volume
- Total volume in room temperature
That's it.
If you have these right your concentration will be correct every time. It really is that simple.
So, let's forget about this powder displacement nonsense. And regarding carrier, just make sure you have enough. Top it off to desired final volume AFTER it reaches room temperature, otherwise it will shrink and concentration will increase. Then filter into a sterile vial.
Done.
You need:
- Powder weight
- Solvent volume
- Total volume in room temperature
That's it.
If you have these right your concentration will be correct every time. It really is that simple.
So, let's forget about this powder displacement nonsense. And regarding carrier, just make sure you have enough. Top it off to desired final volume AFTER it reaches room temperature, otherwise it will shrink and concentration will increase. Then filter into a sterile vial.
Done.