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A question about filtering section!

xeaven

xeaven

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Hello,

I am going to cook 250g Test e with gso,bb and ba
For filtering I heard that it will be too difficult to filter 1liter batch by syringe, but is it really?
I am going to use 0.22 micron pvdf.
I wonder that If it is possible to use sterile filters again and again, I mean I will draw 10ml from the batch to syringe and filter it then again and again untill 1 liter done with one filter.

Or second plan
First I will filter it by vacuum pump and 0.22 micron pvdf(non sterile) membran
and then
I will filter it second time by 0.22 micron pvdf sterile syringe filter because I can't find sterile pvdf bottle top filters.

Can anyone help about that?

This filtering section is really expensive
 
Actually I was going to use pes but people says pes for water base solutions, pvdf for oil. That makes me confused.
 
For personal use I would use the non sterile bottle top into a sterile receiving flask. The stuff coming out of the membrane will be sterile, making minimal contact with anything not sterile and 2% BA will likely mitigate any pathogens to speak of.

No way would I use syringe filters for 100cc let alone a liter. PES is fine for that mix, imo.
 
but the membrane isn't sterile, is it still fine ?
 
I use 60ml syringes in a caulkgun which holds somewhere around 45ml when placed in the gun. A few gentle squeezes provides enough pressure to keep it flowing for a few minutes using grapeseed oil as the carrier and an 18g attached to the filter. Bottle top would be easier for bigger batches but I have no problem brewing 50ml or even 100 with this method.

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Also I am thinking that, I may filter some hydrogen peroxide and isopropyl alcohol before filtering gear,
Because I sterilize my rubber stoppers by this way. Why not for filters?
 
dont run that crap through your filter man... Just buy a sterile filter with media bottle attached.. and if you use a caulk gun be careful you don't blow out the filter.


1141-rls_9.jpg

It comes sealed in sterile packaging
 
Syringe filtering 1 liter!! OMFG hahaha that's pure torture. I want to see a video..
 
tone said:
Syringe filtering 1 liter!! OMFG hahaha that's pure torture. I want to see a video..
Click to expand...
I was reading this and I was getting ready to post until the guy he would be there for 3 Days filtering 1000ml through a syringe filter, but I see somebody already told them to use a bottle top filter. Which if you do do not use a fox autofill spend the few extra dollars and get the Nalgene rapid flow .45un, and make sure you connect it to a gl45 glass media bottle. I don't know if someone has already said this but also go to Harbor Freight and buy yourself a $15 hand pump and make sure you get the nylon filter with the bottle top filter unfortunately Nalgene doesn't make a pvdf. Good luck brother let us know if you have any questions we're all here to help and learn..

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Thanks for all replies. I decided to buy millipore stericup streil ready to use bottle top. They are 12Q and selling in a package. So that means I have enough filter for rest of my life lol
 
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