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Syringe filter
- Thread starter Btheman
- Start date
- Joined
- Apr 3, 2015
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Copy & paste ahead...
HPLC Samples
The majority of syringe filter users are filtering sample prior to injection into and HPLC system. An HPLC vial has a maximum usable volume of 1.8mls typically and should be filled with approx 1ml maximum of sample.
Assuming a moderately clean sample, syringe filters can filter the following*approximate*volumes of sample:
4mm: 1-2ml
17mm: 1-10ml (could go as high as 50ml)
30mm: 1-50ml (could go as high as 100ml)
Obviously, the more particulates in the sample, the lower the volume which can be filtered.
But, for the majority of HPLC samples, a 4mm or 17mm is not only good enough, but also less expensive than using a 30mm filter. Instead of trying to save money by moving to a cheaper, less reliable filter, it makes more sense to move to a smaller size filter, save money, and still achieve the performance.
So why do so many people use 25mm or 30mm filters? The simple answer is that they are often just easier to use. If you have plenty of sample then filtering enough for an HPLC sample is really easy, even for more particulate samples. The available membrane area means that the sample is easy to push through and the filter is less lilley to block up. For really difficult samples, then the addition of a glass fibre pre-filter can help a lot as well, and these are only available in larger filter sizes
HPLC Samples
The majority of syringe filter users are filtering sample prior to injection into and HPLC system. An HPLC vial has a maximum usable volume of 1.8mls typically and should be filled with approx 1ml maximum of sample.
Assuming a moderately clean sample, syringe filters can filter the following*approximate*volumes of sample:
4mm: 1-2ml
17mm: 1-10ml (could go as high as 50ml)
30mm: 1-50ml (could go as high as 100ml)
Obviously, the more particulates in the sample, the lower the volume which can be filtered.
But, for the majority of HPLC samples, a 4mm or 17mm is not only good enough, but also less expensive than using a 30mm filter. Instead of trying to save money by moving to a cheaper, less reliable filter, it makes more sense to move to a smaller size filter, save money, and still achieve the performance.
So why do so many people use 25mm or 30mm filters? The simple answer is that they are often just easier to use. If you have plenty of sample then filtering enough for an HPLC sample is really easy, even for more particulate samples. The available membrane area means that the sample is easy to push through and the filter is less lilley to block up. For really difficult samples, then the addition of a glass fibre pre-filter can help a lot as well, and these are only available in larger filter sizes
worldweary19
Registered
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- Mar 1, 2015
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- Reaction score
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I use 1 filter for 50ml and still purge the filter...
Sent from my SM-G930V using Tapatalk
Sent from my SM-G930V using Tapatalk
It also depends on what kind of material DE filter is made out of personally I feel like I get more through a pvdf whatman zap cap filter and then any other brand or any other material such as nylon PES GDX. There are many things to take into account besides just the pore size.
Has anyone else tried a GDX Walkman filter?
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