Liquidex
Registered
Im pretty sure some of you came accross with vial of gear from unknown underground lab, and it look dusty, yet your too cheap to throw away.
Well heres the cheap way to tell if gear is sterile or not.
Have you ever seen on TV scientist swaping samples on petri dish at laboratory and few days later bacteria grows on plate? Thats what we are going after here.
Heres what you need to do.
1.Get one of these sterile glass petri-dish. MAKE SURE THIS IS STERILE OR WHOLE TEST RESULTS WILL BE MESSED UP!!!!
You can sterilize by dipping the dish into 10% bleach and leanse with alcohol, then dry by placing up side down in dry sterile place or put in the oven (370 degree C) for an hour.
2.Prepare agar powder by placing it in the microwave with the appropriate amount of water. The label should provide specific directions although 6.9 grams to 500 ml of water (or just a little more than two cups) is standard. For agar tablets, 10 tablets dissolved in two cups of water is standard. Melt the agar powders by letting the solution boil for several minutes.
Pour the agar into the plates, fill about 1/8 of inch, then let it solidify. After solidifying process, it should look like this.
3. Now we can take syringe, draw the solution from the vial we are testing. Squirt about .25ml into the agar surface. Make sure you do this with lid covering!!! We dont want the agar to expose into open air too much!!! Single drop of saliva, .0000001mm of dust and your breath will mess this up!!
4. Now place the petri-dish into warm places without air movement. Tape the side of plate so that air doesnt get in and lid intact with plate.
5. Check in about 24 to 48 hours. If your gear was sterile, there will be no bacterial colonies growing, and if the gear was dirty, the plate should look something like this.
There, now you know how to test your self if the gear is sterile. Dont just grab unknown bottle and inject. You can seriously get infected if its contaminated. Play smart, and know how things work.
Well heres the cheap way to tell if gear is sterile or not.
Have you ever seen on TV scientist swaping samples on petri dish at laboratory and few days later bacteria grows on plate? Thats what we are going after here.
Heres what you need to do.
1.Get one of these sterile glass petri-dish. MAKE SURE THIS IS STERILE OR WHOLE TEST RESULTS WILL BE MESSED UP!!!!
You can sterilize by dipping the dish into 10% bleach and leanse with alcohol, then dry by placing up side down in dry sterile place or put in the oven (370 degree C) for an hour.
2.Prepare agar powder by placing it in the microwave with the appropriate amount of water. The label should provide specific directions although 6.9 grams to 500 ml of water (or just a little more than two cups) is standard. For agar tablets, 10 tablets dissolved in two cups of water is standard. Melt the agar powders by letting the solution boil for several minutes.
Pour the agar into the plates, fill about 1/8 of inch, then let it solidify. After solidifying process, it should look like this.
3. Now we can take syringe, draw the solution from the vial we are testing. Squirt about .25ml into the agar surface. Make sure you do this with lid covering!!! We dont want the agar to expose into open air too much!!! Single drop of saliva, .0000001mm of dust and your breath will mess this up!!
4. Now place the petri-dish into warm places without air movement. Tape the side of plate so that air doesnt get in and lid intact with plate.
5. Check in about 24 to 48 hours. If your gear was sterile, there will be no bacterial colonies growing, and if the gear was dirty, the plate should look something like this.
There, now you know how to test your self if the gear is sterile. Dont just grab unknown bottle and inject. You can seriously get infected if its contaminated. Play smart, and know how things work.
