so i've noticed many people get referred to http://peptidecalculator.com/calculator.php to calculate dosage for peptides. as far as i can tell there is a fundamental flaw in the calculation; the calculator does not take into account the volume of the peptide.
let's say you have 5mg of peptide and you add 5cc of water, making a solution of water and peptide. the solution will be greater than 5cc because the 5mg of peptide must displace a certian volume of water. if something has a mass (5mg in this example) then it must have a volume.
don't believe me? try this; measure 200ml water, then add 50g sugar to the water to make a solution. now measure the volume of solution. it should be around 250ml, because the sugar takes up space.
so, if we were to pretend for a minute that we didn't measure the volume of the solution after the sugar was added to the water, and assume that the amount of solvent (water) and solution are the same (pretending that sugar does not take up space), and then try to calculate the amount of sugar in 20ml of solution, you would be left with 5 grams of sugar. right?
NO. THAT IS WRONG.
20ml of 250ml is 8%. 8% of 50g sugar is 4g. NOT 5g.
to get an accurate dose you need to either know the amount of bac water your peptide displaces, OR measure the volume of solution (assuming you know how much peptide you have). i don't know about y'all, but i'd rather measure the volume of solution.
the calculator will only give an accurate measurement if you put in the total volume of the solution where it says "Bacteriostatic water"
so, if you've been following the calculator and basing your dosages purely on what it says, you're probable shorting your self. just a heads up.
let's say you have 5mg of peptide and you add 5cc of water, making a solution of water and peptide. the solution will be greater than 5cc because the 5mg of peptide must displace a certian volume of water. if something has a mass (5mg in this example) then it must have a volume.
don't believe me? try this; measure 200ml water, then add 50g sugar to the water to make a solution. now measure the volume of solution. it should be around 250ml, because the sugar takes up space.
so, if we were to pretend for a minute that we didn't measure the volume of the solution after the sugar was added to the water, and assume that the amount of solvent (water) and solution are the same (pretending that sugar does not take up space), and then try to calculate the amount of sugar in 20ml of solution, you would be left with 5 grams of sugar. right?
NO. THAT IS WRONG.
20ml of 250ml is 8%. 8% of 50g sugar is 4g. NOT 5g.
to get an accurate dose you need to either know the amount of bac water your peptide displaces, OR measure the volume of solution (assuming you know how much peptide you have). i don't know about y'all, but i'd rather measure the volume of solution.
the calculator will only give an accurate measurement if you put in the total volume of the solution where it says "Bacteriostatic water"
so, if you've been following the calculator and basing your dosages purely on what it says, you're probable shorting your self. just a heads up.